James L Wynn

James L Wynn, M.D.

Professor

Department: MD-PEDS-NEONATOLOGY
Business Phone: (352) 273-8985
Business Email: james.wynn@peds.ufl.edu

About James L Wynn

Dr. James Lawrence Wynn is a Professor of Pediatrics and Pathology, Immunology, and Experimental Medicine. He earned his medical degree and completed his clinical training in Pediatrics and Neonatal-Perinatal Medicine at the University of Florida (2002-2008). He served as faculty at Duke University and Vanderbilt University before joining UF Health in July of 2015. His research program centers on the investigation of sepsis in newborn and premature infants. He is a principle investigator on three active NIH R01 awards (2 clinical; 1 basic science). He has published 77 peer-reviewed manuscripts, reviews, book chapters, and editorials. He is an Associate Editor for Pediatric Research, has served as reviewer for the NIH, the Bill and Melinda Gates Foundation, USAID, and over 35 scientific journals. He has mentored over 20 trainees ranging from undergraduate to post-doctoral level.

Board Certifications

  • Neonatal – Perinatal Medicine
    American Board of Pediatrics

Clinical Profile

Specialties
  • Pediatrics
Subspecialties
  • Neonatal-Perinatal Medicine
Areas of Interest
  • Neonatal sepsis

Research Profile

The Wynn laboratory (http://wynn.research.pediatrics.med.ufl.edu) is focused on the investigation of neonatal-specific innate immune cellular function and inflammatory signaling during sepsis as well as development of novel therapeutic immunomodulatory strategies aimed at improving sepsis outcomes. Sepsis represents a significant clinical problem in the developmentally immature preterm neonate where attack rates may reach 60 percent with a 40 percent rate of death/major disability in developed countries. We employ both preclinical mechanistic investigations in association with observational human studies to improve our understanding of the neonatal-specific host response to sepsis. We use a wide variety of molecular and genetic techniques to interrogate the immune response via in vivo, ex vivo, and in vitro approaches. Applications of our work include improving the accuracy of sepsis diagnostic methods, identification of prognostic and clinical stratification markers, and discovery of potential opportunities for translational interventions aimed at improving infection-related outcomes.

Open Researcher and Contributor ID (ORCID)

0000-0001-7052-4836

Areas of Interest
  • Neonatal Sepsis

Publications

2021
Absence of relationship between serum cortisol and critical illness in premature infants
Archives of Disease in Childhood – Fetal and Neonatal Edition. 106(4):408-412 [DOI] 10.1136/archdischild-2020-319970.
2021
Contribution of Concurrent Comorbidities to Sepsis-Related Mortality in Preterm Infants ≤ 32 Weeks of Gestation at an Academic Neonatal Intensive Care Network.
American journal of perinatology. [DOI] 10.1055/a-1675-2899. [PMID] 34674193.
2021
Correction: Neonatal sepsis: need for consensus definition, collaboration and core outcomes.
Pediatric research. 90(1) [DOI] 10.1038/s41390-020-01221-8. [PMID] 33122842.
2021
Criteria for Pediatric Sepsis-A Systematic Review and Meta-Analysis by the Pediatric Sepsis Definition Taskforce.
Critical care medicine. [DOI] 10.1097/CCM.0000000000005294. [PMID] 34612847.
2021
Evaluation of the Neonatal Sequential Organ Failure Assessment and Mortality Risk in Preterm Infants With Late-Onset Infection.
JAMA network open. 4(2) [DOI] 10.1001/jamanetworkopen.2020.36518. [PMID] 33538825.
2021
Hourly Kinetics of Critical Organ Dysfunction in Extremely Preterm Infants
American Journal of Respiratory and Critical Care Medicine. [DOI] 10.1164/rccm.202106-1359oc.
2021
Maximum vasoactive-inotropic score and mortality in extremely premature, extremely low birth weight infants
Journal of Perinatology. 41(9):2337-2344 [DOI] 10.1038/s41372-021-01030-9. [PMID] 33712712.
2021
Multicenter Validation of the Neonatal Sequential Organ Failure Assessment Score for Prognosis in the Neonatal Intensive Care Unit.
The Journal of pediatrics. 236:297-300.e1 [DOI] 10.1016/j.jpeds.2021.05.037. [PMID] 34022247.
2021
Neonatal sepsis definitions from randomised clinical trials.
Pediatric research. [DOI] 10.1038/s41390-021-01749-3. [PMID] 34743180.
2021
Sepsis and Mortality Prediction in Very Low Birth Weight Infants: Analysis of HeRO and nSOFA.
American journal of perinatology. [DOI] 10.1055/s-0041-1728829. [PMID] 33971672.
2020
A neonatal sequential organ failure assessment score predicts mortality to late-onset sepsis in preterm very low birth weight infants.
Pediatric research. 88(1):85-90 [DOI] 10.1038/s41390-019-0517-2. [PMID] 31394566.
2020
Application of metabolomics to neonatal meningitis.
Pediatric research. 88(2):155-156 [DOI] 10.1038/s41390-020-0954-y. [PMID] 32396924.
2020
BCG vaccination–induced emergency granulopoiesis provides rapid protection from neonatal sepsis
Science Translational Medicine. 12(542) [DOI] 10.1126/scitranslmed.aax4517. [PMID] 32376769.
2020
Cell-free hemoglobin increases inflammation, lung apoptosis, and microvascular permeability in murine polymicrobial sepsis.
PloS one. 15(2) [DOI] 10.1371/journal.pone.0228727. [PMID] 32012200.
2020
Challenges in developing a consensus definition of neonatal sepsis.
Pediatric research. 88(1):14-26 [DOI] 10.1038/s41390-020-0785-x. [PMID] 32126571.
2020
Exploring Clinically-Relevant Experimental Models of Neonatal Shock and Necrotizing Enterocolitis.
Shock (Augusta, Ga.). 53(5):596-604 [DOI] 10.1097/SHK.0000000000001507. [PMID] 31977960.
2020
Neonatal sepsis: need for consensus definition, collaboration and core outcomes.
Pediatric research. 88(1):2-4 [DOI] 10.1038/s41390-020-0850-5. [PMID] 32193517.
2020
Pediatric Sepsis Definition-A Systematic Review Protocol by the Pediatric Sepsis Definition Taskforce.
Critical care explorations. 2(6) [DOI] 10.1097/CCE.0000000000000123. [PMID] 32695992.
2019
A Controlled Mouse Model for Neonatal Polymicrobial Sepsis.
Journal of visualized experiments : JoVE. (143) [DOI] 10.3791/58574. [PMID] 30741260.
2019
Early onset and hospital acquired neonatal sepsis associated with high mortality.
The Journal of pediatrics. 204:320-323 [DOI] 10.1016/j.jpeds.2018.10.075. [PMID] 30579470.
2019
Prolonged early antimicrobials in ELBWs: too much for too little.
Pediatric research. 85(7):929-930 [DOI] 10.1038/s41390-019-0360-5. [PMID] 30836378.
2019
Rethinking management of neonates at risk of sepsis.
Lancet (London, England). 394(10195):279-281 [DOI] 10.1016/S0140-6736(19)31627-7. [PMID] 31354128.
2019
Toward the elimination of bias in Pediatric Research.
Pediatric research. 86(6):680-681 [DOI] 10.1038/s41390-019-0583-5. [PMID] 31533126.
2018
Adjuvant pretreatment with alum protects neonatal mice in sepsis through myeloid cell activation.
Clinical and experimental immunology. 191(3):268-278 [DOI] 10.1111/cei.13072. [PMID] 29052227.
2018
Chorioamnionitis, IL-17A, and fetal origins of neurologic disease.
American journal of reproductive immunology (New York, N.Y. : 1989). 79(5) [DOI] 10.1111/aji.12803. [PMID] 29271527.
2018
Complete Genome Sequence of the Multidrug-Resistant Neonatal Meningitis Escherichia coli Serotype O75:H5:K1 Strain mcjchv-1 (NMEC-O75).
Microbiology resource announcements. 7(10) [DOI] 10.1128/MRA.01043-18. [PMID] 30533615.
2018
Cutting Edge: IL-1α and Not IL-1β Drives IL-1R1–Dependent Neonatal Murine Sepsis Lethality
The Journal of Immunology. 201(10):2873-2878 [DOI] 10.4049/jimmunol.1801089. [PMID] 30305325.
2018
Enteral Feeding as an Adjunct to Hypothermia in Neonates with Hypoxic-Ischemic Encephalopathy.
Neonatology. 113(4):347-352 [DOI] 10.1159/000487848. [PMID] 29510382.
2018
IL-17 in neonatal health and disease.
American journal of reproductive immunology (New York, N.Y. : 1989). 79(5) [DOI] 10.1111/aji.12800. [PMID] 29243317.
2018
Impact of toll-like receptor 4 stimulation on human neonatal neutrophil spontaneous migration, transcriptomics, and cytokine production.
Journal of molecular medicine (Berlin, Germany). 96(7):673-684 [DOI] 10.1007/s00109-018-1646-5. [PMID] 29808244.
2018
Limited achievement of NIH research independence by pediatric K award recipients.
Pediatric research. 84(4):479-480 [DOI] 10.1038/s41390-018-0056-2. [PMID] 29904137.
2018
Name and Characteristics of National Institutes of Health R01-Funded Pediatric Physician-Scientists: Hope and Challenges for the Vanishing Pediatric Physician-Scientists.
JAMA pediatrics. 172(3):297-299 [DOI] 10.1001/jamapediatrics.2017.4947. [PMID] 29340570.
2018
Progress in the management of neonatal sepsis: the importance of a consensus definition.
Pediatric research. 83(1-1):13-15 [DOI] 10.1038/pr.2017.224. [PMID] 29019470.
2018
Validation of the Sepsis MetaScore for Diagnosis of Neonatal Sepsis.
Journal of the Pediatric Infectious Diseases Society. 7(2):129-135 [DOI] 10.1093/jpids/pix021. [PMID] 28419265.
2018
Why are preterm newborns at increased risk of infection?
Archives of Disease in Childhood – Fetal and Neonatal Edition. 103(4):F391-F394 [DOI] 10.1136/archdischild-2017-313595. [PMID] 29382648.
2017
Editorial: The Neonatal Immune System: A Unique Host-Microbial Interface.
Frontiers in pediatrics. 5 [DOI] 10.3389/fped.2017.00274. [PMID] 29312907.
2017
Genome-wide association study of sepsis in extremely premature infants
Archives of Disease in Childhood – Fetal and Neonatal Edition. 102(5):F439-F445 [DOI] 10.1136/archdischild-2016-311545. [PMID] 28283553.
2017
Immunological Defects in Neonatal Sepsis and Potential Therapeutic Approaches.
Frontiers in pediatrics. 5 [DOI] 10.3389/fped.2017.00014. [PMID] 28224121.
2017
Impact of Early-Life Exposures to Infections, Antibiotics, and Vaccines on Perinatal and Long-term Health and Disease.
Frontiers in immunology. 8 [DOI] 10.3389/fimmu.2017.00729. [PMID] 28690615.
2017
Neutrophil chemotaxis and transcriptomics in term and preterm neonates.
Translational research : the journal of laboratory and clinical medicine. 190:4-15 [DOI] 10.1016/j.trsl.2017.08.003. [PMID] 28873345.
2017
Oral colostrum priming shortens hospitalization without changing the immunomicrobial milieu.
Journal of perinatology : official journal of the California Perinatal Association. 37(1):36-41 [DOI] 10.1038/jp.2016.161. [PMID] 27684425.
2017
Survival, bacterial clearance and thrombocytopenia are improved in polymicrobial sepsis by targeting nuclear transport shuttles.
PloS one. 12(6) [DOI] 10.1371/journal.pone.0179468. [PMID] 28628637.
2017
Timing of Multiorgan Dysfunction among Hospitalized Infants with Fatal Fulminant Sepsis.
American journal of perinatology. 34(7):633-639 [DOI] 10.1055/s-0036-1597130. [PMID] 27923248.
2017
Unique transcriptomic response to sepsis is observed among patients of different age groups
PLOS ONE. 12(9) [DOI] 10.1371/journal.pone.0184159. [PMID] 28886074.
2016
Defining neonatal sepsis.
Current opinion in pediatrics. 28(2):135-40 [DOI] 10.1097/MOP.0000000000000315. [PMID] 26766602.
2016
Heart rate characteristic index monitoring for bloodstream infection in an NICU: a 3-year experience.
Archives of disease in childhood. Fetal and neonatal edition. 101(4):F329-32 [DOI] 10.1136/archdischild-2015-309210. [PMID] 26518312.
2016
Histological chorioamnionitis shapes the neonatal transcriptomic immune response
Early Human Development. 98:1-6 [DOI] 10.1016/j.earlhumdev.2016.06.001. [PMID] 27318328.
2016
Lethal neonatal meningoencephalitis caused by multi-drug resistant, highly virulent Escherichia coli.
Infectious diseases (London, England). 48(6):461-6 [DOI] 10.3109/23744235.2016.1144142. [PMID] 27030919.
2016
Matrix Metalloproteinase-8 Augments Bacterial Clearance in a Juvenile Sepsis Model.
Molecular medicine (Cambridge, Mass.). 22:455-463 [DOI] 10.2119/molmed.2016.00058. [PMID] 27506554.
2016
Targeting IL-17A attenuates neonatal sepsis mortality induced by IL-18.
Proceedings of the National Academy of Sciences of the United States of America. 113(19):E2627-35 [DOI] 10.1073/pnas.1515793113. [PMID] 27114524.
2015
Il-18 Increases Murine Neonatal Sepsis Mortality Via Il-17a
Shock. 43(6, 1):89-90
2015
Neonatal CD71+ Erythroid Cells Do Not Modify Murine Sepsis Mortality.
Journal of immunology (Baltimore, Md. : 1950). 195(3):1064-70 [DOI] 10.4049/jimmunol.1500771. [PMID] 26101326.
2015
Postnatal Age Is a Critical Determinant of the Neonatal Host Response to Sepsis
Molecular Medicine. 21:496-504 [DOI] 10.2119/molmed.2015.00064. [PMID] 26052715.
2015
Trif-Dependent Innate Immune Activation Is Critical for Survival To Neonatal Gram-Negative Sepsis
Journal of Immunology. 194(3):1169-1177 [DOI] 10.4049/jimmunol.1302676. [PMID] 25548220.
2014
A prime time for trained immunity: innate immune memory in newborns and infants.
Neonatology. 105(2):136-41 [DOI] 10.1159/000356035. [PMID] 24356292.
2013
Determining population and developmental pharmacokinetics of metronidazole using plasma and dried blood spot samples from premature infants.
The Pediatric infectious disease journal. 32(9):956-61 [DOI] 10.1097/INF.0b013e3182947cf8. [PMID] 23587979.
2013
Early administration of oropharyngeal colostrum to extremely low birth weight infants.
Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine. 8(6):491-5 [DOI] 10.1089/bfm.2013.0025. [PMID] 23805944.
2013
Effects of low-dose dopamine on urine output in normotensive very low birth weight neonates.
Journal of perinatology : official journal of the California Perinatal Association. 33(8):619-21 [DOI] 10.1038/jp.2013.20. [PMID] 23448938.
2013
Role of innate immunity in neonatal infection.
American journal of perinatology. 30(2):105-12 [DOI] 10.1055/s-0032-1333412. [PMID] 23297181.
2013
Use of a computerized C-reactive protein (CRP) based sepsis evaluation in very low birth weight (VLBW) infants: a five-year experience.
PloS one. 8(11) [DOI] 10.1371/journal.pone.0078602. [PMID] 24244325.
2012
Loss of Myd88 Or Trif Does Not Impact Survival To Neonatal Polymicrobial Sepsis
Shock. 37:29-30
2012
Very low birth weight neonates who survive early-onset sepsis do not have an increased risk of developing late-onset sepsis.
Early human development. 88(11):905-9 [DOI] 10.1016/j.earlhumdev.2012.07.009. [PMID] 22840605.
2011
B cells enhance early innate immune responses during bacterial sepsis.
The Journal of experimental medicine. 208(8):1673-82 [DOI] 10.1084/jem.20101715. [PMID] 21746813.
2011
Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants.
PloS one. 6(12) [DOI] 10.1371/journal.pone.0027858. [PMID] 22174751.
2011
Critical role for CXC ligand 10/CXC receptor 3 signaling in the murine neonatal response to sepsis.
Infection and immunity. 79(7):2746-54 [DOI] 10.1128/IAI.01291-10. [PMID] 21518789.
2010
Mechanisms and regulation of the gene-expression response to sepsis.
Pediatrics. 125(6):1248-58 [DOI] 10.1542/peds.2009-3274. [PMID] 20478944.
2010
The Role of Ip-10/Cxcr3 in the Neonatal Immune Response To a Septic Insult
Shock. 33
2010
Type I interferon signaling in hematopoietic cells is required for survival in mouse polymicrobial sepsis by regulating CXCL10.
The Journal of experimental medicine. 207(2):319-26 [DOI] 10.1084/jem.20091959. [PMID] 20071504.
2009
Blockade of Ip-10 Inhibits Adjuvant-Induced Protection During Neonatal Sepsis
Shock. 31:9-10
2009
Potential of Immunomodulatory Agents for Prevention and Treatment of Neonatal Sepsis
Journal of Perinatology. 29(2):79-88 [DOI] 10.1038/jp.2008.132. [PMID] 18769381.
2009
Probiotic microbes: do they need to be alive to be beneficial?
Nutrition reviews. 67(9):546-50 [DOI] 10.1111/j.1753-4887.2009.00226.x. [PMID] 19703261.
2008
Defective innate immunity predisposes murine neonates to poor sepsis outcome but is reversed by TLR agonists.
Blood. 112(5):1750-8 [DOI] 10.1182/blood-2008-01-130500. [PMID] 18591384.
2008
Defective Neonatal Innate Immunity Causes Poor Sepsis Outcome, But Is Overcome By Select Toll-Like Receptor Agonists
Shock. 29:96-97
2008
Polymicrobial Sepsis Activates B Cells Through a Type I Interferon Dependent But Myd88 Independent Pathway
Shock. 29
2008
Stromal Cell Derived Factor-1 Mediates Myeloid Derived Suppressor Cell Expansion During Polymicrobial Sepsis
Shock. 29
2007
Increased mortality and altered immunity in neonatal sepsis produced by generalized peritonitis.
Shock (Augusta, Ga.). 28(6):675-683 [PMID] 17621256.
View on: PubMed
2007
MyD88-dependent expansion of an immature GR-1(+)CD11b(+) population induces T cell suppression and Th2 polarization in sepsis.
The Journal of experimental medicine. 204(6):1463-74 [PMID] 17548519.
View on: PubMed
2007
Treatment with GITR agonistic antibody corrects adaptive immune dysfunction in sepsis.
Blood. 110(10):3673-81 [PMID] 17690255.
View on: PubMed
2006
Increased natural CD4+CD25+ regulatory T cells and their suppressor activity do not contribute to mortality in murine polymicrobial sepsis.
Journal of immunology (Baltimore, Md. : 1950). 177(11):7943-9 [PMID] 17114466.
View on: PubMed
1998
Cyclosporine, but not FK506, selectively induces renal and coronary artery smooth muscle contraction.
Surgery. 123(4):456-60 [PMID] 9551073.
View on: PubMed
1982
Outcome for newborn babies declined admission to a regional neonatal intensive care unit.
Archives of disease in childhood. 57(5):334-7 [PMID] 7092287.
View on: PubMed
Balance between protective and pathogenic immune responses to pneumonia in the neonatal lung enforced by gut microbiota
. [DOI] 10.1101/2021.09.27.461705.

Grants

Aug 2019 ACTIVE
Salivary Diagnostics for Sepsis Screening in the Neonate
Role: Principal Investigator
Funding: TUFTS MEDICAL CENTER via NATL INST OF HLTH NICHD
Jun 2018 ACTIVE
Modifiable Determinants of Mortality in Neonatal Sepsis
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS
Aug 2017 ACTIVE
Microfluidic Assessment of Clinical Outcomes in Preterm Newborns
Role: Principal Investigator
Funding: NATL INST OF HLTH NICHD
Jul 2015 – Mar 2018
The Role of the Inflammasome in Neonatal Sepsis
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS

Education

Fellowship – Neonatal- Perinatal Medicine
2008 · University of Florida
Residency – Pediatrics
2005 · University of Florida
Internship – Pediatrics
2003 · University of Florida
Medical Degree
2002 · University of Florida

Teaching Profile

Courses Taught
2018
MDC7401 Senior Pediatric Clk
2015
GMS6029 Brain Journal Club

Contact Details

Phones:
Business:
(352) 273-8985
Emails: